PC-12分化（大鼠腎上腺嗜鉻細(xì)胞瘤） 
產(chǎn)品品牌：ATCC
價(jià)格：詢(xún)價(jià)
我公司擁有國(guó)內(nèi)的細(xì)胞服務(wù)技術(shù)、先進(jìn)的儀器設(shè)備和專(zhuān)業(yè)的ELISA檢測(cè)試劑盒， 生物素標(biāo)記、熒光素標(biāo)記、酶標(biāo)記等，廣泛用于多種分析研究與技術(shù)測(cè)定。

產(chǎn)品說(shuō)明：
Remove and discard culture medium.
 Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
 Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

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